中国肿瘤生物治疗杂志2012，Vol.19Issue(5)：502-507,6.DOI:10.3872/j.issn.1007-385X.2012.05.007
RNAi沉默RON基因对人结肠癌HT-29细胞侵袭和耐药性的抑制作用
Silencing RON gene expression via RNA interference inhibits human colon carcinoma HT-29 cell invasion and drug resistance
摘要
Abstract
Objective;To investigate the effect of receptor tyrosine kinase recepteur d' origine nantais {RON) gene silencing on the invasion and anticancer drug resistance of human colon carcinoma HT-29 cells. Methods; RNAi lentiviral vector targeting RON gene ( Lv-RON-siRNA) was constructed. The efficiency of Lv-RON-siRNA on RON gene silence and RON protein level in HT-29 cells were detected by real-time PCR and Western blotting, respectively. The effects of Lv-RON-siRNA on invasion and drug resistance of HT-29 cells were observed by Transwell assay and ATP-TCA ( ATP-tumor chemosensitivity assay). Results: The silencing effect of Lv-RON-siRNA on RON gene expression in HT-29 cells reached 70%. Compared with the control group, the invasion of HT-29 cells in Lv-RON-siRNA infection group was decreased (0. 97 ± 0. 07 vs 1. 29 ± 0. 08 , P < 0. 05 ). The values of IC90 and IC50 of HT-29 cells infected with Lv-RON-siRNA to 5-FU were ( 14. 28 ± 1. 34)μg/ml and ( 8. 93 ± 1. 2)μg/ml, respectively. The IC90 and IC50 of HT-29 cells infected with Lv-RON-siRNA to cisplatin (DDP) were (1.91 ±0.22)μg/ml and (0.64 ±0.07)μg/ml, respectively, and were significantly lower than those in the control group (P <0. 01). Silencing RON gene expression can decrease the invasion ability of HT-29 cells and increase the sensitivity of HT-29 cells to 5-FU and DDP.关键词
RON基因/HT-29细胞/RNAi/侵袭/耐药Key words
RON gene/ HT-29 cell/ RNAi/ invasion/ drug resistance分类
医药卫生引用本文复制引用
毛联钢,李克强,卓文莹,戴晓宇,余永明,冯伟云,乐东海..RNAi沉默RON基因对人结肠癌HT-29细胞侵袭和耐药性的抑制作用[J].中国肿瘤生物治疗杂志,2012,19(5):502-507,6.基金项目
宁波市自然科学基金资助项目(No.2008A610082). （No.2008A610082）
