中国肿瘤生物治疗杂志2012，Vol.19Issue(5)：513-516,4.DOI:10.3872/j.issn.1007-385X.2012.05.009
硼替佐米提高耐药K562/ADM细胞对NK细胞杀伤的敏感性及其机制
Bortezomib increases cytotoxic sensitivity of drug-resistant K562/ADM cells to NK cells and its mechanisms
摘要
Abstract
Objective;To investigate the effects of bortezomib on the cytotoxic sensitivity of drug-resistant K562/ADM cells to natural killer ( NK) cells and the underlying mechanisms. Methods; The expressions of MICA protein and mRNA on K562/ADM target cells before and after incubation with bortezomib were detected by flow cytometry and real-time PCR, respectively. The cytotoxic sensitivity of K562/ADM cells treated with or without bortezomib to NK cells was measured by LDH releasing assay. Results: The expression rates of MICA protein on K562/ADM cells incubated with bortezomib increased from (17.03 ±4.94)% to (23.77 +5.26)% (P<0.05). The mRNA expression of MICA on K562/ADM cells treated with bortezomib increased (2.03 ±0.33) times. At the E: T ratio of 10: 1 and 20: 1 , the cytotoxic sensitivity of K562/ADM cells to NK cells increased from (23. 22 ± 3. 03) % and (30. 30 ±0. 74) % in untreated cells to (33.69 ± 1.28)% and (41.40 ± 1. 97)% in bortezomib-treated cells, respectively, showing significant differences (P<0.05). Bortezomib can up-regulate the MICA expression in K562/ADM cells and thus may enhance the cytotoxicity of NK cells against K562/ADM cells.关键词
硼替佐米/NK细胞/MHCⅠ类链相关分子A(MICA)/耐药K562/ADM细胞Key words
bortezomib/ nature killer cell/ major histocompatibility complex class I chain-related molecule A (MIVA) / drug-resistant K562/ADM cell分类
医药卫生引用本文复制引用
张虹丽,李亮亮,曾鹏云,邓黎黎,张连生,柴晔,岳玲玲,吴重阳,李莉娟,郝正栋..硼替佐米提高耐药K562/ADM细胞对NK细胞杀伤的敏感性及其机制[J].中国肿瘤生物治疗杂志,2012,19(5):513-516,4.基金项目
甘肃省科技支撑计划资助项目(No.0804NKCA115). （No.0804NKCA115）
