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MicroRNA-34a contributes to the protective effects of glucagon-like peptide-1 against lipotoxicity in INS-1 cells

HAN Yu-bing WANG Min-nan LI Qiang GUO Lin YANG Yu-mei LI Peng-jie WANG Wei ZHANG Jin-chao

中华医学杂志（英文版）2012，Vol.125Issue(23)：4202-4208,7.

中华医学杂志（英文版）2012，Vol.125Issue(23)：4202-4208,7.DOI:10.3760/cma.j.issn.0366-6999.2012.23.008

MicroRNA-34a contributes to the protective effects of glucagon-like peptide-1 against lipotoxicity in INS-1 cells

HAN Yu-bing ¹WANG Min-nan ²LI Qiang ¹GUO Lin ²YANG Yu-mei ³LI Peng-jie ¹WANG Wei ²ZHANG Jin-chao¹

作者信息

1. Department of Endocrinology and Metabolism, Second Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang 150086, China
2. Province Key Laboratory of Hormonal and Endocrine Diseases, Second Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang 150086, China
3. Department of Endocrinology and Metabolism, Second Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang 150086, China
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摘要

Abstract

Background Glucagon-like peptide-1 (GLP-1) reduces fatty acid-induced beta-cell lipotoxicity in diabetes; however,the explicit mechanisms underlying this process are not fully understood.This study was designed to investigate the involvement of microRNA,which regulates gene expression by the sequence-specific inhibition of mRNA transcription in the GLP-1 mediation of beta-cell function.Methods The cell viability and apoptosis were determined using an methyl thiazoleterazolium (MTT) assay and flow cytometry.The expression of genes involved in beta-cell function,including microRNA-34a and sirtuin 1,were investigated using real-time PCR.The underlying mechanisms of microRNA-34a were further explored using cell-transfection assays.Results A 24-hours incubation of INS-1 cells with palmitate significantly decreased cell viability,increased cell apoptosis and led to the activation of microRNA-34a and the suppression of sirtuin 1.A co-incubation with GLP-1 protected the cells against palmitate-induced toxicity in association with a reduction in palmitate-induced activation of microRNA-34a.Furthermore,palmitate-induced apoptosis was significantly increased in cells that were infected with microRNA-34a mimics and decreased in cells that were infected with microRNA-34a inhibitors.Conclusion MicroRNA-34a is involved in the mechanism of GLP-1 on the modulation of beta-cell growth and survival.

关键词

Glucagon-like peptide-1/palmitate/apoptosis/MicroRNA/sirtuin 1

Key words

Glucagon-like peptide-1/palmitate/apoptosis/MicroRNA/sirtuin 1

引用本文复制引用

HAN Yu-bing,WANG Min-nan,LI Qiang,GUO Lin,YANG Yu-mei,LI Peng-jie,WANG Wei,ZHANG Jin-chao..MicroRNA-34a contributes to the protective effects of glucagon-like peptide-1 against lipotoxicity in INS-1 cells[J].中华医学杂志（英文版）,2012,125(23):4202-4208,7.

基金项目

This work was supported by a grant from the National Natural Science Foundation of China (No.81170744). （No.81170744）

中华医学杂志（英文版）

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ISSN：0366-6999

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