中华医学杂志（英文版）2012，Vol.125Issue(23)：4245-4248,4.DOI:10.3760/cma.j.issn.0366-6999.2012.23.015
Interleukin-18 and-12 synergistically enhance cytotoxic functions of tumor-infiltrating lymphocytes
Interleukin-18 and-12 synergistically enhance cytotoxic functions of tumor-infiltrating lymphocytes
CHEN Zhi-fen 1ZHOU Rui 2XIA Bing 3DENG Chang-sheng1
作者信息
- 1. Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China
- 2. Clinical Center of Intestinal & Colorectal Diseases of Hubei Province, Wuhan, Hubei 430071, China
- 3. Key Laboratory of Intestinal & Colorectal Diseases of Hubei Province, Wuhan, Hubei 430071, China
- 折叠
摘要
Abstract
Background The role of tumor-infiltrating lymphocytes (TILs) in the immunopathogenesis of individual cancer is not clear and is a challenge for anti-tumor immunotherapy.This study aimed to investigate the effects of interleukin (IL)-18 and-12 on cytotoxic functions of TILs.Methods TILs from postoperative gastric cancer patients were costimulated with IL-18 and IL-12.SGC-7901 tumor cells were pre-incubated with TILs and subcutaneously injected into BALB/C SCID mice.The function of TILs was evaluated by measuring tumor sizes in tumor-bearing mice,T helper (Th)1 (tumor necrosis factor (TNF)-α,interferon (IFN)-Y) and Th2 cytokine levels (IL-10 and IL-4) in serum and cytotoxicity of mouse natural killer (NK) and CD8+ T cells.Results IL-18 and IL-12 synergistically inhibited the growth of SGC-7901 cells in vivo and significantly extended the survival rate of SGC-7901-bearing mice (66.7％ vs.13.7％,P ＜0.01).Moreover,TILs could promote the secretion of TNF-α and IFN-Y ((130.34±7.65) vs.(210.63±12.31) pg/ml,P ＜0.01; (14.23±1.97) vs.(30.52±2.12) pg/ml,P ＜0.01),and downregulate IL-10 and IL-4 secretion ((103.72±11.21) vs.(61.36±5.41) pg/ml,P=0.021; (49.36±4.67) vs.(28.48±3.86)pg/ml,P=0.024).Conclusion IL-18 and IL-12 can synerqistically enhance cytotoxic functions of TILs from human gastric cancer.关键词
interleukin-18/interleukin-12/tumor-infiltrating lymphocyte/gastric cancerKey words
interleukin-18/interleukin-12/tumor-infiltrating lymphocyte/gastric cancer引用本文复制引用
CHEN Zhi-fen,ZHOU Rui,XIA Bing,DENG Chang-sheng..Interleukin-18 and-12 synergistically enhance cytotoxic functions of tumor-infiltrating lymphocytes[J].中华医学杂志（英文版）,2012,125(23):4245-4248,4.
