肿瘤防治研究2012，Vol.39Issue(9)：1064-1069,6.DOI:10.3971/j.issn.1000-8578.2012.09.006
柠檬酸钠联合三氧化二砷体外抗人胃癌细胞及其作用机制
Anti-tumor Effects and Mechanism of Arsenic Trioxide in Combination with Sodium Citrate in Gastric Cancer Cells in vitro
摘要
Abstract
Objective To study the anti-tumor effects of citrate combined with arsenic trioxide on human gastric cancer cell in vitro and possible mechanism. Methods Citrate (final concentration of 5 mmol / L) and (or) different concentrations of As2O3 (final concentration was 5 or 10 μmol / L) treated in vitro cultured gastric cancer cell line SGC-7901 and BGC-803. Flow cytometry was used to detect apoptosis and cell cycle changes. The apoptosisrelated gene Bcl-2 and Mcl-1 expressions were identified by RT-PCR to observe. Results Citrate combining with As2 O3 had a stronger inhibition function on human gastric cancer cell line compared with citrate or As2O3 (P<0. 05) alone. Compared with blank group, the cell cycle distribution was arrested at G2/ M phase, and the proportion of G0/G1 phase cell decreased and G2/M phase increased (P<0. 05). The expression of bcl-2 and mcl-1 was significantly decreased(P<0. 05). Conclusion Citrate combining with As2O3 had the synergistic effects on gastric cancer cell. The mechanism might be related to cell cycle blocking and apoptosis-inducing effects through bcl-2 and mcl-1 genes.关键词
胃癌/柠檬酸钠/三氧化二砷/凋亡/Bcl-2基因/Mcl-1基因Key words
Gastric cancer/Sodium citrate/Arsenic trioxide/Apoptosis/Bcl-2/Mcl-1分类
医药卫生引用本文复制引用
郑步平,张晓东,陆云飞..柠檬酸钠联合三氧化二砷体外抗人胃癌细胞及其作用机制[J].肿瘤防治研究,2012,39(9):1064-1069,6.基金项目
广西自然科学基金资助项目(0832118) （0832118）
