肿瘤防治研究2012，Vol.39Issue(9)：1070-1075,6.DOI:10.3971/j.issn.1000-8578.2012.09.007
慢病毒介导ITGB4 shRNA对H460SM细胞增殖的抑制作用
Inhibition of Lentivirus-mediated ITGB4 shRNA on H460SM Cell Proliferation
摘要
Abstract
Objective To investigate the effect of β4 integrin gene silencing on the proliferation of human lung carcinoma variant cell line H460SM was investigated in vitro to further provide new targets for early diagnosis and treatment of non-small cell lung cancer. Methods β4 integrin gene expression was detected by quantitative reverse transcriptase PCR, and the cellular morphology was identified by microscope. Tumor cells growth was detected by MTS assay in vitro. Furthermore, the changes of cell cycle and apop-tosis were analyzed by flow cytometry. Results Compared with negative control of shRNA-transduced H460SM cells(H460SM-NS) ,β4 integrin gene expressions in two different H460SM cells with integrin β4-specific shRNA ( H460SM68 and H460SM71) were statistically down-regulated ( P < 0. 01). The growth rates of H460SM cells with stably expressing integrin β4-specific shRNA significantly were decreased compared with negative control (P<0. 05). Knockdown of β4 integrin by two different shRNAs led to significantly increase in apoptotic rate as compared with negative control(P<0. 01). The proportion of H460SM cells with integrin β4-specific shRNA in the G0/G1 phase was significantly higher than that of negative control,suggesting that the cell proliferation was inhibited as blocking at G1 /S phase(P<0. 05). PI value of H460SM cells with integrin β4-specific shRNA was also lower than that of negative control(P <0. 05). Conclusion Knockdown of β4 integrin expression in non-small cell lung cancer cells could inhibit in vitro cell proliferation. Furthermore, β4 integrin shRNA could induced cell apoptosis in H460SM cells. The possible mechanism of H460SM cell growth inhibition resulted from β4 integrin might be related to cell cycle arrest.关键词
非小细胞肺癌/β4整合素/细胞增殖/细胞凋亡/细胞周期Key words
Non-small cell lung cancer/β4 integrin/Cell proliferation/Cell apoptosis/Cell cycle分类
医药卫生引用本文复制引用
陈艳,周永春,金从国,伍治平,刘馨,陈晓群,李佳,王熙才..慢病毒介导ITGB4 shRNA对H460SM细胞增殖的抑制作用[J].肿瘤防治研究,2012,39(9):1070-1075,6.基金项目
国家自然科学基金资助项目(81060177) （81060177）
云南省应用基础研究基金资助项目(2009CD181) （2009CD181）
中国人事部留学人员科技活动项目择优资助启动基金资助项目(人社厅[2010]412号) （人社厅[2010]412号）
云南省卫生科技资助项目(2009NS079) （2009NS079）
