中山大学学报（自然科学版）2012，Vol.51Issue(6)：107-111,5.
一种用于黄曲霉高质量总RNA提取方法与应用
A Method for Total RNA Extraction from Fungus Aspergillus flavus and Its Application
摘要
Abstract
The rapidly developing molecular techniques require the ability to extract high quality RNA from Aspergillus flavus. The cell wall structure and endogenous RNase activity in A. flavus are often the main obstacles for obtaining RNA with high quality. In this paper, TES hot phenol method was applied to obtain total RNA from A. flavus, and the total RNA was further used in RT-PCR ( Reverse translation-polymerase chain reaction) and RNA-sequencing. The results showed that the TES hot phenol method can be used to get a good quality of total RNA from this filamentous fungus as templates for following studies, such as RT-PCR and RNA sequencing.关键词
黄曲霉/RNA提取/TES热酚法/RT-PCR/转录组测序Key words
Aspergillus flavus/ RNA extraction/ TES hot phenol method/ RT-PCR/ RNA-Sequencing分类
生物科学引用本文复制引用
林剑青,王成成,肖春华,陈国华,贺竹梅..一种用于黄曲霉高质量总RNA提取方法与应用[J].中山大学学报（自然科学版）,2012,51(6):107-111,5.基金项目
国家自然科学基金资助项目(31170044) （31170044）
