中山大学学报（医学科学版）2012，Vol.33Issue(6)：716-722,7.
矢车菊素-3-葡萄糖苷通过降低STAT3活化抑制TNF-α诱导的小鼠血管平滑肌细胞增殖
Cyanidin-3-glucoside Inhibits TNF-α-Induced Mouse Vascular Smooth Muscle Cells Proliferation through Suppression of STAT3 Activation
摘要
Abstract
[Objective] To investigate the role of Cyanidin-3-glucoside (C3G) in TNF-α-inducd proliferation of vascular smooth muscle cells (VSMC) and the possible mechanisms. [Methods] VSMC derived from C57BL/6J mouse aorta were obtained from ATCC and cultured in vitro. The influence of C3G on TNF-α-induced cell proliferation was assessed by cell proliferation assay. The TNF-α-induced production of reactive oxygen species (ROS) in VSMC was evaluated by Dihydroethidium (DHE) staining. The mRNA level of NADPH Oxidase Activator 1 (NoxA1) was measured by real-time PCR. The protein levels of NoxA 1, Signal Transducer and Activator of Transcription 3 (STAT3), Phosphorylated STAT3 and β-actin were semi-quantitatively measured by western blotting. The significance of difference (P < 0.05) was determined by one-way ANOVA using SPSS13.0. [Results] C3G pretreatment inhibited VSMC proliferation induced by TNF-a in a dose and time-dependent manner. Combination of 50 μM C3G and 100 μM apocynin significantly reduced TNF-α mediated production of ROS. Combined usage of C3G and apocynin was more effective in suppressing NoxAl gene expression and STAT3 phosphorylation in VSMC treated by TNF-α than using C3G or apocynin alone. ROS scavenger catalase (2000 U/mL) significantly inhibited TNF-α-induced VSMC proliferation and STAT3 activation. [Conclusions] The mechanism of the anti-proliferaling effect of C3G on TNF-α treated VSMC was probably through the repression of NoxAl induced ROS production and the subsequent inhibition of STAT3 activation.关键词
矢车菊素-3-葡萄糖苷/信号转导与转录激活因子3/活性氧/血管平滑肌细胞/NADPH氧化酶活化蛋白1Key words
cyanidin-3-glucoside/ signal transducer and activator of transcription 3/ reactive oxygen species/ vascular smooth muscle cell/ NADPH oxidase activator 1分类
医药卫生引用本文复制引用
方仕,罗小琴,吴晓滨,麦海妍,彭俊生,卢味,卓淑雨,叶艳彬..矢车菊素-3-葡萄糖苷通过降低STAT3活化抑制TNF-α诱导的小鼠血管平滑肌细胞增殖[J].中山大学学报（医学科学版）,2012,33(6):716-722,7.基金项目
国家"十一·五"科技支撑计划(2008BAD91B00) （2008BAD91B00）
广东省教育部产学研结合项目(2011B090400558) （2011B090400558）
