中山大学学报（医学科学版）2012，Vol.33Issue(6)：728-732,5.
多西紫杉醇联合二烯丙三硫协同诱导前列腺癌细胞凋亡的研究
Synergetic Cell Apoptotic Effect Induced by Docetaxel Combined with Diallyl Trisulfide in Prostate Cancer Cells
摘要
Abstract
[Objective] To study the killing effect of docetaxel combined with diallyl trisulfide (DATS) in prostate cancer cell line PC-3 and further to determine the mechanism. [Methods] PC-3 cells were treated with various concentrations of docetaxel or (and) DATS. Cell viability was estimated using MTT assay. Apoptotic cells were detected following staining with DAPI. The percentage of sub G1 and cell cycle were confirmed by flow cytometry. Protein levels of apoptosis regulating proteins were determined using Western bolt including Bcl-2, Bax, Bcl-xL/xS, cytochrome c, caspase-9, and caspase-3. [Results] Docetaxel or DATS alone could inhibit the proliferation of PC-3 cells. For combination group, the cell viability was decreased to (50.1 ±1.0)% to compare with (67.4 ± 1.0)% of docetaxel at 10 nmol/L and (69.2 ± 1.8)% of DATS at 15 μmol/L separately and there was a synergistic effect between these two drugs. Apoptotic rate and the cell number in S-phase and G-phase of the combination group were higher than other two drugs. The combination treatment could significantly reduce the Bcl-2, Bcl-xL expression and promote the release of cytochrome c and the cleavage of caspase-9 and caspase-3. [Conclusion] The combination of docetaxel with DATS can inhibit the proliferation of prostate cancer cells congenerously by inducing apoptosis, which may be correlated with S-and G2-phase arrest, the down-regulation of Bcl-2, Bcl-xL, the release cytochrome c and the activation of caspase-9 and caspase-3.关键词
前列腺癌/凋亡/多西紫杉醇/二烯丙三硫Key words
prostate neoplasm/ apoptosis/ docetaxel/ diallyl trisulfide分类
医药卫生引用本文复制引用
毛晓鹏,陈俊星,莫承强,庄锦涛,丘少鹏..多西紫杉醇联合二烯丙三硫协同诱导前列腺癌细胞凋亡的研究[J].中山大学学报（医学科学版）,2012,33(6):728-732,5.基金项目
国家自然科学基金(81001146 （）
30872584) （）
教育部新教师基金(20090171120062) （20090171120062）
中央高校基本科研业务费专项资金(11ykpy21) （11ykpy21）
