中山大学学报（医学科学版）2012，Vol.33Issue(6)：775-780,6.
大鼠自体原位肝移植24h后肝内硫氧还蛋白-2的表达和抗氧化能力的变化
Patterns of Alteration to Thioredoxin-2 Expression and Antioxidant Capacity during 24h in Liver Tissue Subjected to Autologous Orthotopic Liver Transplantation in Rats
摘要
Abstract
[Objective] Mitochondria thioredoxin (thioredoxin 2) and its modulated antioxidant enzymes compose the critical endogenous antioxidant system in human body to scavenge reactive oxygen species (ROS). We aimed to investigate the patterns of alteration to thioredoxin2 expression and antioxidants capacity in liver tissue during 24 h after liver transplantation in rats. [Methods] Forty Sprague Dawley (SD) rats were randomly divided into sham group, and 4, 8, 16, and 24 h after autologous orthotopic liver transplantation ( AOLT) group. Rats in sham group only suffered from the process of laparotomy and vascular separation, while in other groups all subjected to AOLT. Liver tissues were collected for detection of pathological changes by light microscope, Trx2 expressions by Western blot, and changes of ROS levels and activities of antioxidants. [Results] AOLT resulted in severe liver injury in rats during 24 hours after reperfusion manifested as significant elevations in liver pathological scores as early as 4 hours after AOLT. There was also obvious oxidative damage that was evidenced with 1.6 to 2.0 fold increases of O2 · or · OH and MDA levels, accompanied with significant decreases (-30%) in the activity of SOD or calalase and a dramatic (-70%) decrease in level of TOC at 4 h and 8 h (P< 0.01 vs. Sham group). All the parameters restored gradually at 16 and 24 hours after AOLT. The expression of Trx2 in liver increased significantly as early as 4 hours and peaked at 24 hours after AOLT (-2.0 fold) (P < 0.05 vs. Sham group). [Conclusion] AOLT could result in severe liver injury, the extent of which coincidence with the oxidant/antioxidant system state manifested as exacerbating first and then alleviating gradually. Up-regulation of Trx2 expression was beneficial to synthesizing antioxidants and further enhancing the antioxidant capacity of liver tissue subjected to ischemia/ reperfusion injury during AOLT.关键词
肝移植/硫氧还蛋白-2/抗氧化酶Key words
liver transplantation/ thioredoxin 2/ antioxidant分类
医药卫生引用本文复制引用
王艳玲,朱琼芳,高婉菱,葛缅,张瑷兰,池信锦,黑子清..大鼠自体原位肝移植24h后肝内硫氧还蛋白-2的表达和抗氧化能力的变化[J].中山大学学报（医学科学版）,2012,33(6):775-780,6.基金项目
广州市科技计划项目(2011J4300056) （2011J4300056）
