中药材2012,Vol.35Issue(7):1062-1065,4.
鸭脚木指纹图谱研究及3种三萜皂苷类化合物的含量测定
Study on the HPLC Fingerprint and Simultaneous Determination of Three Triterpene Glycosides in Schefflera heptaphylla
摘要
Abstract
Objective:To establish the HPLC fingerprint and simultaneously determination three triterpene glycosides in Schefflera heptaphylla. Methods:The separation was carried out on an Agilent Zorbax SB-C18(4. 6 mm × 250 mm ,5μm)column eluted with meth-anol-0. 1% glacial acetic acid as the mobile phase,and samples were detected by an evaporative light scattering detector (ELSD). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was used in data analysis. Results: Schefflera heptaphylla from different regions were similar and the separation of three triterpene glycosides was good. Conclusion: This method is simple and reliable. The HPLC fingerprint and contents of three kinds of triterpene glycosides of Schefflera heptaphylla can be used for their quality control.关键词
鸭脚木/HPLC-ELSD/指纹图谱/三萜皂苷Key words
Schefflera heptaphylla (L ) Frodin/ HPLC-ELSD/ Fingerprint/ Triterpene glycosides分类
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吴春,王国才,段营辉,吴霞,周光雄,叶文才,李药兰..鸭脚木指纹图谱研究及3种三萜皂苷类化合物的含量测定[J].中药材,2012,35(7):1062-1065,4.基金项目
广东省自然科学基金团队项目(8351063201000003) (8351063201000003)