中药材2012，Vol.35Issue(7)：1112-1116,5.
甘草糖提取物对小肠上皮细胞迁移及多胺含量影响的研究
Effect of Polysaccharides from Radix Glycyrrhizae on Migration and Polyamines Contents of IEC-6 Cell
摘要
Abstract
Objective: To study the effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines ( putrescine, spermidine and spermine) contents of IEC-6 cell. Methods: Cell migration model was induced by scratch method in each well, and the polyamines in IEC-6 cell was determined by pre-column derivation high performance liquid chromatography. The polysaccharides inhibited effect on migration and polyamines contents of IEC-6 cells, and on IEC-6 cell migration by DFMO ( a polyamines synthesis inhibitor) and the polyamines contents in the cells were observed. Results:The polysaccharides (50 mg/L or 100 mg/L) was able to promote the cell migration, reverse the cell migration inhibition by DFMO, enhance the IEC-6 cell polyamines ( putrescine, spermidine and spermine) contents in the process of cell migration and reverse the reduction of polyamines ( putrescine,spermidine and spermine) induced by DFMO. Conclusion: The effect of Radix Glycyrrhizae on the gastrointestinal mucosal damage repairing may be related to increasing polyamine content in cells and promoting cell migration.关键词
甘草/糖复合物/多胺/小肠上皮细胞/细胞迁移Key words
Radix Glycyrrhizae/Polysaccharides/Polyamine/IEC-6 cell/Cell migration分类
医药卫生引用本文复制引用
温鹏,随晶晶,李茹柳,赵世清,卢文彪,陈蔚文..甘草糖提取物对小肠上皮细胞迁移及多胺含量影响的研究[J].中药材,2012,35(7):1112-1116,5.基金项目
国家自然科学基金项目(30772753,81173254) （30772753,81173254）
上海市教育委员会E-研究院建设计划项目(E03008) （E03008）
