中药材2012，Vol.35Issue(7)：1116-1120,5.
青蒿素半乳糖苷的抗肿瘤活性及其机制研究
In vitro Antitumor Activities and Mechanisms of Galactosylated Artemisinin
摘要
Abstract
Objective:To explore the antitumor activities of galactosylated artemisinin in vitro and the related mechanisms. Methods : Apoptosis rate of Hela cell was evaluated by using flow cytometry ( FCM) analysis. The activity of Caspase-3 was examined by col-orimetry. The expression level of p53 ,BAX,Caspase-3 and BCL-2 was determined by Western blotting. Results;The FCM data showed that galactosylated artemisinin induced apoptosis of Hela cell. The activity and expression level of Caspase-3 were significantly increased when treated with galactosylated artemisinin for 36 h and 72 h. The expression of BAX was also increased markedly, while BCL-2 was reduced after treatment with the drug. However,galactosylated artemisinin had no effects on the expression of p53. Conclusion: Galactosylated artemisinin exhibits great antitumor activities,which may be ralated to triggering cytochrome C apoptotic pathway which mediated by BCL-2 family.关键词
青蒿素半乳糖苷/抗肿瘤/p53/BAX/Caspase-3/BCL-2Key words
Galactosylated artemisinin/ Antitumor activity/ p53 / BAX/ Caspanse-3 / BCL-2分类
医药卫生引用本文复制引用
任彦荣..青蒿素半乳糖苷的抗肿瘤活性及其机制研究[J].中药材,2012,35(7):1116-1120,5.基金项目
重庆市教育委员会资助项目(KJ101507) （KJ101507）
