中药材2012，Vol.35Issue(7)：1144-1147,4.
酶法辅助提取补骨脂中总黄酮的工艺研究
Study on the Extraction Process of Total Flavonoids from Psoralea corylifolia by Enzyme-assisted Technique
严优芍 1廖华卫 1郭丽冰1
作者信息
- 1. 广东药学院中药学院,广东广州510006
- 折叠
摘要
Abstract
Objective:To study the optimum extraction process of total flavonoids from Psoralea corylifolia by cellulose-assisted technique. Methods: Based on single-factor experiments, the effects of pH value, temperature of enzymatic hydrolysis, time of enzymatic hydrolysis and enzyme amount on the extraction yields of total flavonoids from Psoralea corylifolia were studied by response surface methodology. Results:The optimum enzyme-assisted extraction process was:pH value 4. 9, temperature 46 ℃ , time 150 min and enzyme amount 7. 2 mg/g,under this condition,the relative error of the observed and predicted values was 1. 16%. Conclusion:The optimum enzyme-assisted extraction process is simple and feasible,the extraction rate of total flavonoids increases by 28% compared with ultrasonic extraction, so it can be used to extract total flavonoids from Psoralea corylifolia.关键词
补骨脂/总黄酮/提取/纤维素酶/响应面法Key words
Psoralea corylifolia L. / Total flavonoids/ Extraction/ Cellulase/ Response surface methodology分类
医药卫生引用本文复制引用
严优芍,廖华卫,郭丽冰..酶法辅助提取补骨脂中总黄酮的工艺研究[J].中药材,2012,35(7):1144-1147,4.
