中药材2012，Vol.35Issue(8)：1234-1237,4.
夏枯草药材HPLC指纹图谱研究
Study on HPLC Fingerprint of Prunella vulgaris
许招懂 1姚江雄 1刘菊妍 2李春 3林丽美4
作者信息
- 1. 广州白云山星群(药业)股份有限公司,广东广州510288
- 2. 广州医药集团有限公司,广东广州510130
- 3. 中国中医科学院中药研究所,北京100700
- 4. 湖南中医药大学,湖南长沙410208
- 折叠
摘要
Abstract
Objective:To establish HPLC fingerprint of Prunella vulgaris and provide evidence for quality control and identification of the Chinese crude drug. Methods; The HPLC method was used,chromatography conditions were Agilent Eclipse XDB-C18 (4. 6 mm × 250 mm,5μm)column with gradient mobile phase of acetonitrile-0. 1% acetic acid,UV detection wavelength was 290 nm and the column temperature was 30 ℃ with the flow rate of 1. 0 raL/min, the sample injection was 10 μL. Results;HPLC fingerprints of 19 samples of Prunella vulgaris were established. 14 common peaks were selected as the fingerprint peaks in all samples. Among the obtained fingerprints, most of the detected peaks were separated effectively. 19 samples had high similarities. Conclusion: The established HPLC fingerprint has desirable accuracy, repeatability and stability, which can be used for one of the quality control methods of Prunella vulgaris.
关键词
夏枯草/HPLC/指纹图谱/质量控制Key words
Prunella vulgaris L. / Fingerprints/ HPLC/ Quality control分类
医药卫生引用本文复制引用
许招懂,姚江雄,刘菊妍,李春,林丽美..夏枯草药材HPLC指纹图谱研究[J].中药材,2012,35(8):1234-1237,4.
