摘要
Abstract
Objective: To investigate the molecular mechanisms underlying the treatment of inflammatory bowel disease ( IBD) by Pulsatilla decoction. Methods: 84 BALB/c mice were randomly divided into ethanol control group, model group, SASP group, Pulsatilla decoction group (further divided into low, middle and high dose group) and zVAD group (n = 12). Intragastric and celiac drug administration were used in each group respectively. The expression of NLRP3, ASC, Caspase-1 ,IL-18 and IL-1β in the colons were detected by fluorescence quantitative RT-PCR, Elisa and immunohistochemistry after treatment respectively. Results: Compared with the model group,SASP group,middle and high dose group could treat IBD effectively (P <0. 01) ,while this effect was restrained in zVAD group ( P < 0. 05 ). In the meantime, middle and high dose group could effectively raise the expression of NLRP3 , ASC, Caspase-1, IL-18 and IL-1β in the colons (P <0. 05) , while this effect was also inhibited in zVAD group (P <0. 05). Conclusion: Pulsatilla decoction is likely to exert their therapeutic effect by activating NLRP3 inflammasome which further promote the formation of the corresponding inflammation factors such as IL-18 and IL-1β.
关键词
白头翁汤/炎症性肠病/NLRP3炎症小体/zVAD-fmkKey words
Pulsatilla decoction/ Inflammatory bowel disease/ NLRP3 inflammasome/ zVAD-fmk分类
医药卫生
