中药材2012，Vol.35Issue(8)：1340-1347,8.
双孢蘑菇多糖超声波辅助提取及体外抗氧化活性
Ultrasonic-assisted Extraction and in vitro Antioxidant Activity of Polysaccharides from Agaricus bisporus
摘要
Abstract
Objective:To optimize ultrasonic-assisted extraction parameters of polysaccharides from Agaricus bisporus and evaluate antioxidant activities of A.bisporus polysaccharides.Methods:Polysaccharides from A.bisporus was extracted by using methods of ultrasonic-assisted hot water lixiviation,ethanol precipitation,Sevag' s deproteination and ethanol precipitation again.Extraction temperature,extraction time,ratio of water to raw material and ultrasonic power were selected in single-factor tests.Based on the single-factor tests,parameters combination for the ultrasonic-assisted extraction of A.bisporus polysaccharides was optimized by using four-factorthree-level orthogonal test.Antioxidant activities (reductive potential,superoxide anion scavenging activity and H2O2 scavenging activity) of A.bisporus polysaccharides were evaluated in vitro.Results:Optimum conditions for the extracting of A.bisporus polysaccharides were extracting temperature 65 ℃,extracting time 40 min,ratio of water to raw material 30 mL/g and ultrasonic power 170 w.Practicing this optimal condition,extraction yield of polysaccharides from A.bisporus was 5.6014％.In crude polysaccharides of A.bisporus,carbohydrates content,determined by applying the phenol-sulfuric acid method,was 75.48％.Polysaccharides of A.bisporus could reduce ferric ion,scavenge superoxide anion and hydrogen peroxide in a dose-dependent manner.Conclusion:Utrasonicassisted extraction could be used in the extracting of A.bisporus polysaccharides.Polysaccharides of A.bisporus,had direct and potent antioxidant activities,might be developed and utilized as natural antioxidant.关键词
双孢蘑菇/多糖/超声波/提取/抗氧化Key words
Agaricus bisporus / Polysaccharides/ Ultrasonic wave/ Extraction / Antioxidation分类
医药卫生引用本文复制引用
乔德亮,赵峰,黄海忠,范纯纯,韩磊..双孢蘑菇多糖超声波辅助提取及体外抗氧化活性[J].中药材,2012,35(8):1340-1347,8.基金项目
安徽省教育厅自然科学研究项目(KJ2010B256) （KJ2010B256）
