组织工程与重建外科杂志2012，Vol.8Issue(5)：241-244,4.DOI:10.3969/j.issn.1673-0364.2012.05.001
毛囊干细胞体外诱导分化为血管内皮细胞的实验研究
Preliminary Study on Inducing Hair Follicle Stem Cells into Vascular Endothelial Cells in Vitro
摘要
Abstract
Objective To investigate the feasibility of inducing human hair FSCs (follicle stem cells) into VECs (vascular endothelial cells) in vitro. Methods The dispase was used to isolate the stem cells and stem cells were cultured in EGM-2 plus 10 ng/mL VEGF and 2 ng/mL bFGF as long as 4th passage as a experimental group, the control group was cultured in EGM-2 without growth factor. The morphological change of each passage was observed and vWF (von Willebrand factor) and CD31 were tested 4 passages later. Results The morphological change to VECs was obviously found in the increasing passage of the induced FSCs, but the phenomenon was not distinct in the control group. Induced FSCs of 4th passage expressed vWF and CD31 in immunocytochemical staining, while little expression was observed in the control groups. FCM showed nearly 80% of induced FSCs expressed vWF and CD31, much higher than the control ones which were lower 5%. RT -PCR further confirmed the experimental group expressed vWF, but not obvious expression in the control group. Conclusion FSCs can be induced to VECs in vitro by EGM-2 plus 10 ng/mL VEGF and 2 ng/mL bFGF.关键词
毛囊干细胞/血管内皮细胞/组织工程Key words
Follicle stem cells/ Vascular endothelial cells/ Tissue engineering分类
生物科学引用本文复制引用
许志成,李宏,张群..毛囊干细胞体外诱导分化为血管内皮细胞的实验研究[J].组织工程与重建外科杂志,2012,8(5):241-244,4.基金项目
国家自然科学基金青年科学基金(81000842). （81000842）
