癌变·畸变·突变2013，Vol.25Issue(1)：13-17,5.DOI:10.3969/j.issn.1004-616x.2013.01.003
131Ⅰ-单克隆抗体对人卵巢癌细胞株OC-3-VGH裸鼠皮下移植瘤的抑制作用
Inhibitory effect of 131I-monoclonal antibody on subcutaneously transplanted human ovarian cancer cell line OC-3-VGH tumor
摘要
Abstract
OBJECTIVE: To explore the inhibitory effect of 131 I-monoclonal antibody on tumor growth of human ovarian cancer cell line OC-3-VGH in nude mice. METHODS: The transplanted human ovarian carcinoma model was established by subcutaneous injection of OC-3-VGH cells in nude mice. The mice were randomly divided into negative control group, CP positive control group (60 mg/kg), high dose (10 mg/kg), low dose (2 mg/kg) group of monoclonal antibody, high dose (10 mg/kg + 125 μCi), medium dose (6 mg/kg + 75 μCi), low dose (2 mg/kg + 25 μ Ci) group of 131 I- monoclonal antibody. Seven groups received continuously intraperitoneal injection for 14 d. Diameters of tumors were measured and nude mice were weighed on d0, d4, d8, dl2, dl5. The animals were killed 24 h after the last treatment. The transplanted tumors were weighed, the inhibitory rate and treatment over control growth ratios were calculated. RESULTS: The high dose monoclonal antibody group, and medium and high dose 131 I-monoclonal antibody groups had significant tumor inhibiting effects in vivo, with treatment over control growth ratios of 54%, 48%, 30% and inhibitory rates of 33.59%, 45.80% and 64.89%, respectively. Compared with the negative control, there was significant difference(P < 0.01). The high dose monoclonal antibody group was compared with the high dose 131I-monoclonal antibody group, showing a statistical difference (P < 0.05). CONCLUSION: Both monoclonal antibody and I- monoclonal antibody groups effectively inhibited the growth of human ovarian carcinoma in vivo. The therapeutic efficiency was enhanced significantly in the high dose 131 I-monoclonal antibody group.关键词
裸鼠/卵巢癌/移植瘤/抑瘤率/放射免疫疗法Key words
nude mice/ovarian cancer/transplanted tumor/tumor inhibitory rate/ radioimmunotherapy分类
医药卫生引用本文复制引用
谢琛静,刘向云,桂博,崇立明,许丽,潘琦,周莉,孙祖越..131Ⅰ-单克隆抗体对人卵巢癌细胞株OC-3-VGH裸鼠皮下移植瘤的抑制作用[J].癌变·畸变·突变,2013,25(1):13-17,5.基金项目
国家"重大新药创制"科技重大专项(2011ZX09301-005) （2011ZX09301-005）
上海市"科技创新行动计划"项目(11140901300 （）
11140901302) （）
