安徽农业大学学报2013，Vol.40Issue(1)：90-94,5.
运动对心肌细胞凋亡及相关基因表达的影响
Effects of exercises on apoptotic and related gene expression in myocardium
摘要
Abstract
In order to investigate the mechanism of myocardial apoptosis by exercise, we studied the effects of different exercise intensities on apoptotic gene Bcl-2, Bax and Caspase-3 in rat myocardium cells. The rats were randomly divided into three groups (control group, aerobic exercise group and overtraining group), the mRNA and protein expressions of apoptotic gene Bax, Bcl-2 and Caspase-3 were observed in myocardium cells by quantitative fluorescent polymerase chain reaction (qPCR) and westernblot. As results, compared with the control group, the mRNA and protein expressions of Bcl-2 both increased in aerobic exercise group which inhibited myocardial apoptosis. However, the decreased Bcl-2 expression and the increased expressions of Bax and Caspase-3 in the overtraining group indicated the promoted apoptosis. In conclusion, overtraining exercise can increase apoptosis in rat cardiac myocytes, and the occurrence of apoptosis may be related to the regulation of gene Bcl-2, Bax and Caspase-3.关键词
运动/凋亡基因/心肌细胞Key words
exercise/ apoptotic gene/ myocardium cell分类
社会科学引用本文复制引用
蒋磊,周振茂,夏美燕,杜世全..运动对心肌细胞凋亡及相关基因表达的影响[J].安徽农业大学学报,2013,40(1):90-94,5.基金项目
安徽省高校省级自然科学研究项目(KJ2012Z170)资助. （KJ2012Z170）
