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吴茱萸组织培养和快速繁育技术研究

唐征 荆赞革 张小玲 罗天宽 刘庆 朱世扬

安徽农业科学Issue(36):17475-17477,3.
安徽农业科学Issue(36):17475-17477,3.

吴茱萸组织培养和快速繁育技术研究

Study on Tissue Culture and Rapid Propagation of Tetradium ruticarpum

唐征 1荆赞革 1张小玲 1罗天宽 1刘庆 1朱世扬1

作者信息

  • 1. 浙南作物育种重点实验室,温州科技职业学院生物工程技术研究所,浙江温州 325006
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摘要

Abstract

[Objective] The paper was to study the tissue culture and rapid propagation of Tetradium ruticarpum. [Method] Using the tender leaf from female plant of Tetradium ruticarpum as explants, the effects of different plant growth regulator and concentration on the induction, proliferation, rooting culture were studied. [ Result] The appropriate adventitious bud inducing medium of Tetradium ruticarpum was MS + 1. 0 mg/L BA +0. 3 mg/L NAA, the inductivity was 74.4% , and the seedling was normal and had no vitrification phenomenon. The best medium for proliferation was MS +2.0 mg/L BA +0. 1 mg/L KT +0. 3 mg/L NAA, the propagation coefficient was 4. 61. The rooting rate could reach to 86% by using 1/2MS +2.0 mg/L NAA as rooting medium, the average rooting number was 5.4. [Conclusion] This study established the Tetradium ruticarpum rapid propagation system, and provided technical support for the industrial seedling production of its excellent female line.

关键词

吴茱萸/组织培养/快速繁殖

Key words

Tetradium ruticarpum/ Tissue culture/ Rapid Propagation

分类

农业科技

引用本文复制引用

唐征,荆赞革,张小玲,罗天宽,刘庆,朱世扬..吴茱萸组织培养和快速繁育技术研究[J].安徽农业科学,2012,(36):17475-17477,3.

安徽农业科学

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