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琼胶分解菌Cellvibrio sp.BR的发酵条件及酶学性质研究

邱荣蓉 刘生峰 何加友 李能章

安徽农业科学Issue(36):17488-17491,4.
安徽农业科学Issue(36):17488-17491,4.

琼胶分解菌Cellvibrio sp.BR的发酵条件及酶学性质研究

Study on the Fermentation Conditions and Agarase's Properties of Agar Degradation Bacteria Cellvibrio sp.BR

邱荣蓉 1刘生峰 2何加友 3李能章3

作者信息

  • 1. 重庆理工大学药学与生物工程学院,重庆 400054
  • 2. 重庆出入境检验检疫局,重庆 400020
  • 3. 西南大学动物科技学院,重庆 400715
  • 折叠

摘要

Abstract

[ Objective ] The aim was to study the fermentation conditions of agar degradation bacteria Celhibrio sp. BR and the enzymatic properties of its agarase. [Method] The optimal fermentation conditions of Celhibrio sp. BR were screened by the optimization of its enzyme production conditions, and then the enzymatic properties of agarase were studied. [Result] The optimal fermentation conditions of Celhibrio sp. BR were that incubated bacteria for 36 h at 25 ℃ and pH 7. 0. The optimal temperature, pH and agar substrate concentration for the agarase were 35 ℃ , 7.0-8.0 and 1. 1% , respectively. And the agarase had significant substrate specificity. [Conclusion] It provided theoretical basis for the further industrial production of agarase.

关键词

琼胶分解菌/琼胶酶/发酵条件/酶学性质

Key words

Agar degradation bacteria/ Agarase/ Fermentation conditions/ Enzymatic properties

分类

农业科技

引用本文复制引用

邱荣蓉,刘生峰,何加友,李能章..琼胶分解菌Cellvibrio sp.BR的发酵条件及酶学性质研究[J].安徽农业科学,2012,(36):17488-17491,4.

基金项目

重庆理工大学青年基金计划(2011ZQ14). (2011ZQ14)

安徽农业科学

0517-6611

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