安徽农业科学2013，Vol.41Issue(1)：33-36,4.
过氧化氢酶高产菌株EIM-70的鉴定及产酶培养基的优化
Identification of Catalase-producing Strain EIM-70 and Its Optimization of Submerged Fermentation Mediums
摘要
Abstract
[Objective]The aim was to identify a high catalase-producing strain EIM-70 and optimize its submerged fermentation mediums. [ Method] The strain EIM-70 was identified by the morphological analysis and phylogenetic analysis of 16S rDNA sequence; the optimal carbon and nitrogen sources for EIM-70 on catalase production were obtained by single-factor experiments, the Plackett - Burman design was adopted to screen out the significant related factors based on this, the optimal fermentation medium was obtained by the steepest ascent search experiment and response surface methodology. [Result] The strain EIM-70 was identified as Bacillus subitilis; the optimized medium for catalase production was glucose 19.25 g/L, NaNO39.25 g/L, FeSO4 · 7H2O 2.46 × 10-3 g/L, MgSO4 · 7H20 0.50 g/L, Na2HPO4 · 12H2O9.25 g/L, KH2PO4 0.60 g/L and 2° malt extract, in this medium, the catalase activity of Bacillus subitilis EIM-70 was up to 6 927.45 U/ml and 1. 87 times higher than control. [Conclusion] It provided foundation for the industrialized production of catalase.关键词
过氧化氢酶/枯草芽孢杆菌/Plackett-Burman设计/响应面分析Key words
Catalase/ Bacillus subitilis/ Plackett - Burman design/ Response surface analysis分类
农业科技引用本文复制引用
杨鹤,蔡少丽,李力,张爱平,黄建忠..过氧化氢酶高产菌株EIM-70的鉴定及产酶培养基的优化[J].安徽农业科学,2013,41(1):33-36,4.基金项目
福建省科技攻关重大项目(2005Q007). （2005Q007）
