安徽农业科学2013，Vol.41Issue(1)：107-109,3.
N+离子束注入诱变选育桑黄菌株的研究
Study on Breeding Phellinus igniarius by N+ Implantation
全卫丰 1汪洁 1刘广建 1薛璟1
作者信息
- 1. 江苏省苏微微生物研究有限公司,江苏无锡214063
- 折叠
摘要
Abstract
[Objective]To breed Phellinus igniarius and improved P. igniarius yield by N+ implantation. [Method] The optimum dosage of ion implantation was obtained through research on survival rate and mutation rate of P. igniarius spore after induction of N + implantation. After mutation, antagonistic test combined with analysis of esterase isozymes to verify strain mutation, with mycelial morphology, growth rate, liquid fermentation mycelial biomass and extracellular polysaccharide yield as parameters to screen variant strain. [Result] The appropriate parameters of the ion implantation mutagenesis P. igniarius were determined; implantation dosage 2.00 x 10 ions/cm , injection energy 20 KeV. A high-yielding strain of P. igniarius called PI 126 was obtained, whose biomass and polysaccharide production are 23.7 g/L and 7. 6 g/L respectively, and the mutant genetic traits are stable after ten generation. [Conclusion] Low energy ion beam implantation is feasible for breeding of P. igniarius.关键词
离子注入/桑黄/育种Key words
Ion implantation/ Phellinus igniarius/ Breeding分类
农业科技引用本文复制引用
全卫丰,汪洁,刘广建,薛璟..N+离子束注入诱变选育桑黄菌株的研究[J].安徽农业科学,2013,41(1):107-109,3.
