安徽农业科学2013，Vol.41Issue(3)：961-962,1022,3.
呋喃唑酮代谢物人工抗原的合成及半抗原偶联比的测定
Synthesis of Furazolidone Metabolite Antigen and Determination of Hapten Coupling Ratio
摘要
Abstract
[Objective] To synthesis the metabolite of furazolidone and determine the coupling ratio of hapten. [Method] AOZ after carboxyl modification was coupled to HSA via an acid anhydride action. The result was confirmed by using UV spectrum analysis and the coupling ratio of small molecular antigen and carrier protein was determined. [Result] AOZ, the metabolite of furazolidone, had been successfully synthesised, and it was coupled with HSA after carboxyl modification. The coupling ratio was identified as 1:4 after determination. [Conclusion] UV spectrum analysis is simple and reliabile way to determine the coupling ratio of small molecular antigen.关键词
呋喃唑酮代谢物/AOZ/抗原合成/偶联比Key words
Furazolidone metabolite/ AOZ/ Antigen synthesis/ Coupling ratio分类
农业科技引用本文复制引用
曾磊,周常义,苏国成..呋喃唑酮代谢物人工抗原的合成及半抗原偶联比的测定[J].安徽农业科学,2013,41(3):961-962,1022,3.基金项目
福建省海洋渔业局重点项目(201101100003). （201101100003）
