安徽农业科学2013，Vol.41Issue(3)：1038-1040,3.
大苞鞘石斛种子萌发过程中形态变化研究
Research on Morphogenesis during Seed Germination of Dendrobium wardianum
陈娜 1方炎明 2程磊1
作者信息
- 1. 毫州职业技术学院药学院,安徽毫州236800
- 2. 南京林业大学森林资源与环境学院,江苏南京210037
- 折叠
摘要
Abstract
[Objective] To study the morphology variation of Dendrobium wardianum during seed germination process. [Method] Seeds of D. wardianum were cultured on medium (MS + 6-BA (0.5 - 1.0) mg/L + NAA 0. 1 mg/L) , and through electron microscopic observation, morphological changes during germination were researched and discussed. [Result] The results showed that seed germination experienced from the pale yellow to pale green; embryo began to expand and broken coat; formation of green PLBs; produced the first leaf primordium; presented obvious root end; formed the lobules. The electron microscopic observation showed that there was a small round spherical embryo in the seed of D. wardianum; seed coat had thickened ring patterns. There were two forms of embryogenesis: ① the direct way: seeds germinated to the formation of PLBs and further differentiated into tube seedlings; ② the indirect pathway: seeds germinated to the formation of a low proportion of callus, and then differentiated into PLBs or directly differentiated forming buds. That the enlargement of growth and the elongation of ra-dicel was the obvious bipolar nature of mature PLBs. [Conclusion] The morphology variation of D. wardianum during seed germination was studied, which can provide basis for future development and utilization of D. wardianum.关键词
大苞鞘石斛(Dendrobium wardianum Warner.)/形态发生/种子/原球茎Key words
Dendrobium wardianum/ Morphogenesis/ Seed/ Protocorm - like bodies (PLBs)分类
农业科技引用本文复制引用
陈娜,方炎明,程磊..大苞鞘石斛种子萌发过程中形态变化研究[J].安徽农业科学,2013,41(3):1038-1040,3.
