安徽农业科学2013，Vol.41Issue(3)：1121-1122,1125,3.
三硝基甲苯对斑马鱼组织ATP酶·乳酸脱氢酶和谷胱甘肽活性的影响
Effects of Trinitrotoluene on Activities of ATP Enzyme, Lactate Dehydrogenase and Glutathione from Zebra Fish Tissue
摘要
Abstract
[Objective] The aim was to explore effects of Trinitrotoluene ( TNT) on activities of ATP enzyme, lactate dehydrogenase (LDH) and glutathione (GSH ) of Danio rerio tissue so as to provide reference for evaluation on toxicity effect of TNT. [Method] Effects of TNT of different concentrations (0.5, 1.0, 2.0 and 4.0 mg/L) on activities of ATP enzyme from liver and gill tissues, LDH from head tissue and GSH from liver tissue of D. rerio were determined by using toxicity test method. [Result] The activity of ATP enzyme from liver and gill tissues decreased with the increase of TNT mass concentration; the activity of GSH from liver tissue increased with the increase of TNT mass concentration ; the activity of LDH from head tissue decreased with the increase of TNT mass concentration. The variation of enzyme activity over time was the same as different concentration but changed slowly. [Conclusion] It can better evaluate the toxic effects of TNT to use the activities of enzymes from D. rerio liver and gills as loxicological indicators.关键词
TNT/斑马鱼/ATP酶/LDH/GSHKey words
Trinitrotoluene/ Danio rerio/ATP enzyme/Lactate dehydrogenase/Glutathione分类
农业科技引用本文复制引用
谢松,付兴亮,曲瑾,王佳,卢胜涛,柳峰松..三硝基甲苯对斑马鱼组织ATP酶·乳酸脱氢酶和谷胱甘肽活性的影响[J].安徽农业科学,2013,41(3):1121-1122,1125,3.基金项目
河北省自然科学基金项目(C2010000256,C2011201027) （C2010000256,C2011201027）
河北大学自然科学基金项目(2011218). （2011218）
