安徽农业科学2013，Vol.41Issue(3)：1142-1144,3.
中华鳖和杂交鳢血清免疫球蛋白免疫印迹和酶联免疫分析
Analysis of Immunoglobulin in the Serum of Trionyx sinensis and Ophiocephalus argus Cantor (♂) × Ophiocephalus maculatus(♀)by Western blot and ELISA
摘要
Abstract
[Objective] The research aimed to study the homology and content of serum immunoglobulin in Trionyx sinensis and Ophiocephalus argus Cantor (♂ ) × Ophiocephalus maculatus (♀). [Method] The serum of T. sinensis and O. argus Cantor (♂) × O. maculatus (♀) was sampled to cultivate with 25 commodities secondary antibodies for Western blot and ELISA analysis. [Result] The serum of T. sinensis and O. argus Cantor (♂ ) × O. maculatus(♀) reacted with six kinds of secondary antibodies(rabbit anti-chicken IgM, rabbit anti-pig IgG, rabbit anti-chicken IgG, rabbit anti-rat IgM, rabbit anti-monkey IgG and goat anti-human IgG)for Western blot. The serum immunnoglobulin level of T. sinensis and O. argus Cantor (♂ ) × O. maculatus(♀) was determined by using ELISA kits of chicken IgG,chicken IgM,swine IgG,rat IgM,mongkey IgG and human IgG. The serum immunnoglobulin concentrations of T. sinensis and O. argus Cantor (♂) × O. maculatus(♀) were 28.5 -215.4 and 24.5 - 201.5 μg/L respectively. [Conclusion] This study could enrich the specific immune system research of T. sinensis and 0. argus Cantor (♂) × O. maculatus(♀) and it was of certain guiding significance for the healthy breeding of T. sinensis and O. argus Cantor (♂) × O. maculatus( ♀).关键词
中华鳖/杂交鳢/免疫球蛋白/免疫印迹/酶联免疫Key words
T. Sinensis/ O. argus Cantor (♂) × O. maculatus(♀)/ Immunoglobulin/ Western blot/ ELISA分类
农业科技引用本文复制引用
刘凯,朱丽敏,蔡丽娟,王宇希,谢楠,沈理..中华鳖和杂交鳢血清免疫球蛋白免疫印迹和酶联免疫分析[J].安徽农业科学,2013,41(3):1142-1144,3.基金项目
浙江省科技厅公益技术研究农业项目(2011C22098) （2011C22098）
杭州市科技重大创新项目(20083212A04). （20083212A04）
