安徽医科大学学报2013，Vol.48Issue(1)：1-4,4.
乳源免疫调节肽诱导人食管癌Eca-109细胞凋亡的研究
Immunomodulating peptide from β-casein induced apoptosis in human esophageal carcinoma cell line Eca-109
摘要
Abstract
Objective To investigate the effect of immunomodulating peptide from (3-casein( PGPIPN ) on the proliferation and apoptosis of human esophageal carcinoma Eca-109 cells. Methods The effect of PGPIPN on Eca-109 cells were evaluated by means of MTT assay. Cells apoptosis was detected by the flow cytometrie assay( FCM ) and agarose gel electrophoresis; immunocytochemistry was used to analyze the expression of survivin and caspase-3 in cells during apoptosis induced by PGPIPN. Results PGPIPN could significantly inhibit the proliferation of Eca-109 cells, which had significant dose-dependent relationships. Its inhibition effect contributed to the induced apoptosis; The study showed that survivin proteins expressed and caspase-3 proteins increased in Eca-109 cells. Conclusion PGPIPN can induce apoptosis of Eca-109 cells in vitro. The mechanism of this apoptotic effect is closely related to the survivin proteins and caspase-3 proteins.关键词
乳源免疫调节肽/Eca-109细胞/细胞凋亡Key words
PGPIPN/ Eca-109 cells/ apoptosis分类
医药卫生引用本文复制引用
顾芳,秦宜德,桂丽,王巍,唐宜桂..乳源免疫调节肽诱导人食管癌Eca-109细胞凋亡的研究[J].安徽医科大学学报,2013,48(1):1-4,4.基金项目
国家自然科学基金(编号:30872992) （编号:30872992）
2011年安徽医科大学校基金(编号:0108027201) （编号:0108027201）
