安徽医科大学学报2013，Vol.48Issue(1)：21-25,5.
左甲状腺素和维生素E对甲状腺功能减退症大鼠心肌损伤的影响
L-T4 and vitamin E intervention on myocardial injury in hypothyroid rats
摘要
Abstract
Objective To investigate the myocardial enzymes, myocardial pathology and oxidative stress status in propylthiouracil-induced hypothyroidism rats and examine the effect of levothyroxine, vitamin E( VitE ) and both supplementation on this experimental model. Methods Male Sprague Dawley rats were randomly divided into normal control group, model group, T4 treatment group, VitE treatment group, T4 + VitE combination therapy group. Serum T3 , T4, TSH levels, serum myocardial enzymes, serum and myocardial superoxide dismutase ( SOD ) activity and malondialdehyde ( MDA ) content were measured. To contrast each group of myocardial cell morphology through hematoxylin and eosin ( H&E ) staining. Results CD Compared with normal control group, T3 and T4 levels were significantly decreased and TSH level was significantly elevated in model group and VitE treatment group ( P <0. 05 ). Compared with model group, T3 and T4 levels were significantly elevated and TSH level was significantly decreased in T4 treatment group and T4 + VitE combination therapy group ( P < 0. 05 ). There was no statistical difference in T3, T4 and TSH levels between model group and VitE treatment group. (2) Compared with normal control group,CK,LDH and AST levels were significantly elevated in model group ( P <0. 05 ). Compared with model group, they were significantly decreased in T4 treatment group, VitE treatment group and T4 + VitE combination therapy group ( P < 0. 05 ), the CKMB difference was not statistically significant. (3) Serum SOD and LDH, AST, CK, CKMB was a significant correlation. (4) HE stain microscope observation showed that compared with control group, there were some mucous changes in myocardium of model group. Compared with model group, the condition of myocardial injury improved in T4 treatment, VitE treatment and T4 + VitE treatment group. (5) Compared with control group, serum and myocardial MDA levels in model group increased significantly ( P < 0. 05 ), serum SOD activity in model group decreased significantly ( P < 0. 05 ), between myocardial SOD activity in each group the difference was not statistically significant. Conclusion Levothyroxine, VitE and both treatment of hypothyroid-ism in rats can significantly reduce the myocardial enzymes, improve the pathology conditions, which may be related to the improvement of thyroid function and reduction of the level of oxidative stress.关键词
甲状腺功能减退症/心肌酶谱/心肌病理学/左甲状腺素/维生素E/氧化应激Key words
hypothyroidism/ myocardial enzymes/ myocardial pathology/ levothyroxine/ vitamin E/ oxidative stress分类
医药卫生引用本文复制引用
万思源,蔡传元,张艳青,钟兴,方朝晖,潘天荣..左甲状腺素和维生素E对甲状腺功能减退症大鼠心肌损伤的影响[J].安徽医科大学学报,2013,48(1):21-25,5.基金项目
国家中医药管理局重点学科-中医内分泌学科开放基金项目(编号:2011nfmxk009) （编号:2011nfmxk009）
