安徽医科大学学报2013，Vol.48Issue(2)：120-123,4.
血清饥饿诱导胃癌SGC-7901细胞内质网应激介导上皮间质转化的发生
Starvation induced endoplasmic reticulum stress mediated epithelial-mesenchymal transition in SGC-7901 gastric cancer cells
史冠男 1甘惠中 1彭琼 1刘尚全 2戴夫1
作者信息
- 1. 安徽医科大学第三附属医院消化内科,合肥,230061
- 2. 安徽医科大学第三附属医院滨湖医院中心实验室,合肥,230061
- 折叠
摘要
Abstract
Objective To investigate whether starvation induced endoplasmic reticulum stress can mediate epitheli-al-mesenchymal transition in SGC-7901 gastric cancer cells. Methods After SGC-7901 cells were cultured without RPMI 1640 for 0,3 ,6,12,24 h, the expression of GRP78 was detected by RT-PCR and Western blot. Take GRP78 high expression time points, RT-PCR and Western blot test the expression of E-cadherin and N-cadherin. The scratch wound assay was used to assess the invasion and migration levels of SGC-7901 cells. Results After exposure to starvation for 6 h, the expression of GRP78 was increased, the expression of N-cadherin was up-regulated ( P < 0. 05 )and E-cadherin was significantly down-regulated( P < 0. 05 )at the same time. The invasion and migration levels of starvation-treated cells were increased as compared with those of control group. Conclusion Starvation-induced endoplasmic reticulum stress can mediate epithelial-mesenchymal transition in gastric cancer cells and improve their invasion and metastasis.关键词
胃癌/内质网应激/上皮间质转化Key words
gastric cancer/ endoplasmic reticulum stress/ epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
史冠男,甘惠中,彭琼,刘尚全,戴夫..血清饥饿诱导胃癌SGC-7901细胞内质网应激介导上皮间质转化的发生[J].安徽医科大学学报,2013,48(2):120-123,4.
