安徽中医学院学报2013，Vol.32Issue(1)：53-56,4.DOI:10.3969/j.issn.1000-2219.2013.01.020
新藤黄酸对黑色素瘤B16细胞凋亡的作用
Effect of Gambogenic Acid on Apoptosis of Melanoma Cell Line B16
摘要
Abstract
Objective To investigate the effect of gambogenic acid (GNA) on the apoptosis of mouse melanoma cell line B16. Methods B16 cells were cultured -with different concentrations of GNA for 24 h. The morphology of Bl6 cells was observed under an inverted microscope; the inhibitory effect of GNA on the proliferation of Bl6 cells was evaluated by methyl thiazolyl tetrazolium assay; the apoptosis of Bl 6 cells was observed by acridine orange (AO)/ethidium bromide (EB) staining under a fluorescence microscope; the apoptosis rate of Bl 6 cells -was measured by annexin V-fluorescein isothiocyanate (FITC)/propidium i-odide (PI) double staining and flow cytometry. Results After treatment with GNA, the inhibition rate of B16 cells increased as the concentration of GNA rose; the B16 cells stained with AO/EB showed typical morphological changes of apoptosis under the fluorescence microscope; the apoptosis rate of B16 cells increased as the concentration of GNA rose, as measured by flow cytometry. Conclusion GNA can inhibit the proliferation of Bl 6 cells through inducing cell apoptosis, and the inhibitory effect is in a time-dependent and concentration-dependent manner within a certain range of GNA concentrations.关键词
新藤黄酸/B16细胞/细胞增殖/细胞凋亡Key words
gambogenic acid/ B16 cell/ cell proliferation/ cell apoptosis分类
医药卫生引用本文复制引用
张璇,王梅,程卉,苏婧婧,李庆林..新藤黄酸对黑色素瘤B16细胞凋亡的作用[J].安徽中医学院学报,2013,32(1):53-56,4.基金项目
国家自然科学基金项目(81173600) （81173600）
安徽省自然科学基金项目(11040606M190) （11040606M190）
国家科技重大专项-新药创制项目(2009ZX09103-399) （2009ZX09103-399）
