安徽中医学院学报2013，Vol.32Issue(1)：70-72,3.DOI:10.3969/j.issn.1000-2219.2013.01.024
益气养阴方对糖尿病肾病气阴两虚大鼠肾组织Janus激酶/信号转导子和转录激活子通路的影响
Effects of Qi-Supplementing and Yin-Nourishing Prescription on JAK/STAT Pathway in Kidney Tissue of Diabetic Nephropathy Rats with Deficiency of Both Qi and Yin
摘要
Abstract
Objective To measure the expression of Janus kinase/signal transducer and activator of transcription (JAK/STAT) path-way members in the kidney tissue of diabetic nephropathy (DN) rats -with deficiency of both qi and yin (DQY) and to investigate the mechanism by which qi-supplementing and yin-nourishing prescription exerts effects on the syndrome of DQY in DN rats. Methods Thirty-three rats were randomly divided into normal group, DN model group, DN + DQY model group, Western medicine (WM) group, and traditional Chinese medicine (TCM) group. Streptozotocin was used to induce a rat model of DN; Pericarpium Citri Reticulatae Viride, Fructus Aurantii Immaturus, and Radix Aconiti Late-ralis Preparata -were used to induce a rat model of DQY by consuming qi and injuring yin. The expression of JAK1/3 and STAT1 in kidney tissue was measured by immunohistochemistry. Results Compared with the normal group, the DN model group and DN + DQY model group had significantly higher expression of JAK1/3 and STAT1 (P<0. 01). The TCM group and WM group had lower JAK1/3 expression than the DN model group and DN + DQY model group, but there were no significant differences between them (P> 0. 05). The TCM group and WM group had significantly lower STAT1 expression than the DN model group and DN + DQY model group (P<0. 05). Conclusion Qi-supplementing and yin-nourishing prescription can reduce the expression of JAK1/3 and STAT1 in the kidney tissue of DN rats with the syndrome of DQY, thus preventing and treating early renal injury.关键词
糖尿病肾病/Janus激酶/信号转导子和转录激活子(JAK/STAT)/气阴两虚证/益气养阴Key words
diabetic nephropathy/ Janus kinase/signal transducer and activator of transcription (JAK/STAT) / deficiency of both qi and yin/ qi-supplementing and yin-nourishing prescription分类
中医学引用本文复制引用
周雪梅,陈雪功,程立,董昌武,王建青,程维克,张红梅..益气养阴方对糖尿病肾病气阴两虚大鼠肾组织Janus激酶/信号转导子和转录激活子通路的影响[J].安徽中医学院学报,2013,32(1):70-72,3.基金项目
安徽中医学院自然科学研究基金项目(2010zr005B) （2010zr005B）
