北京林业大学学报2013,Vol.35Issue(1):83-87,5.
中国与美国松材线虫体表细菌在线虫体表对峙及对松苗致病性
Colonization and pathogenicity for American and Chinese bacterial strains carried by pine wood nematodes
摘要
Abstract
Bacterial succession on the surface of pine wood nematode (PWN) may have a direct impact on the development of pine wilt disease ( PWD) . The objective of this study was to isolate bacteria from PWN in the United States where PWD was not a serious problem and compared with four bacterial strains ( YG1 , YG2, YG3 , YG4) from China. Fifteen bacterial strains including seven dominant strains ( MG3 , MG4, MG5, MG8, MG9, MG11 and MG12) were isolated from 3 PWN originating in the USA. In competition assays, Four ( MG4, MG5 , MG8 and MG9) of the 15 American bacterial strains better colonized sterile PWN than all Chinese strains tested. These three American strains ( MG5 , MG8 , MG9 ) were further compared with one reference Chinese strain ( YG2) for control efficacy using Pinus thunbergii, P. massoniana,P. elliottii and P. tabuliformis as model systems. MG8 and MG9 provided better control of PWD caused by sterile PWN than YG2. These results show that two American strains MG8 and MG9 have great potential as biological control agents for PWD and deserve further studies.关键词
松材线虫/优势菌/对峙试验/致病性Key words
Bursaphelenchus xylophilus / dominant bacteria/ competition experiment/ pathogenicity分类
农业科技引用本文复制引用
贲爱玲,曾腓力,乔雪娟,郑敬荣,韩正敏..中国与美国松材线虫体表细菌在线虫体表对峙及对松苗致病性[J].北京林业大学学报,2013,35(1):83-87,5.基金项目
林业公益性行业科研专项(20120450)、国家自然科学基金项目(30872026、31170600). (20120450)