草业学报2013，Vol.22Issue(1)：210-216,7.
外源一氧化氮对渗透胁迫下黑麦草幼苗光合和生物发光特性的影响
Effects of exogenous nitric oxide on photosynthetic and bioluminescent characteristics in ryegrass seedlings under osmotic stress
摘要
Abstract
The regulating effect of nitric oxide (NO) on photosynthetic physiological response of herbage under osmotic stress, was studied in a hydroponic experiment. The effect of the exogenous NO donor sodium nitro-prusside (SNP) on the photosynthetic pigment content, gas exchange and chlorophyll fluorescence parameters, and bioluminescence intensity in leaves of ryegrass (Lolium perenne) under osmotic stress simulated by 15% PEG-6000 were investigated. The stress from 100 μmol/L SNP significantly increased photosynthetic pigment contents, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), stomatal limited value (Ls), PSⅡ maximal photochemical efficiency (Fv/Fm) and actual photochemical efficiency (ΦPSⅡ ), but reduced intercellular CO2 concentration (Ci), PS Ⅱ excitation pressure (1-qp), non-photochemical quenching (NPQ), ultra-weak luminescence intensity, fluorescence intensity and phosphorescence intensity in ryegrass seedling leaves. The addition of haemoglobin (Hb), a NO scavenger, reversed the effects of SNP, but application of 100 μmol/L sodium nitrate or nitrite (the decomposition products of NO or its donor SNP) or sodium ferricyanide (an analog of SNP) had no significant alleviation effects on osmotic stress. NO may alleviate the damage of osmotic stress to the leaf photosynthetic apparatus by increasing photosynthetic pigment and light-energy utilization, and decreasing bioluminescent intensity, thus improving photosynthetic efficiency of the ryegrass seedlings under osmotic stress.关键词
一氧化氮/渗透胁迫/光合作用/叶绿素荧光/生物发光/黑麦草Key words
nitric oxide/ osmotic stress/ photosynthesis/ chlorophyll fluorescence/ bioluminescence/ Lolium perenne分类
农业科技引用本文复制引用
刘建新,王金成,王瑞娟,贾海燕..外源一氧化氮对渗透胁迫下黑麦草幼苗光合和生物发光特性的影响[J].草业学报,2013,22(1):210-216,7.基金项目
甘肃省庆阳市科技支撑计划项目(GK098-1-34)资助. （GK098-1-34）
