草业学报2013，Vol.22Issue(1)：315-322,8.
添加外源纤维水解酶对羊草体外瘤胃发酵酶活性及特性的影响
Effects of exogenous fibrolytic enzymes on the enzyme activities and fermentation characteristic of Chinese wildrye by mixed ruminal microorganisms in vitro
摘要
Abstract
Three levels of mixture of cellulase and xylanase supplementations (0, 10. 0 and 50. 0 mg, respectively) and two pretreatment time (0 and 24 h) in triplicate were used to determine the effects of the mixture enzyme on the enzyme activities and fermentation characteristic of Chinese wildrye by mixed ruminal microorganisms in vitro. Fibrolytic enzymes increased (P<0. 05) activities of xylanase and endoglucanase at 0 and 8 h and exoglucanase activity at 0 h postincubation in fermentation fluid. In addition, the enzyme activities increased with exogenous enzyme preparation level. However, the exogenous enzyme preparation did not affect the enzyme activities at 24 and 48 h of incubation. The content of acetic acid at 24 and 48 h, the total volatile fatty acids content at 8, 24, 48 h incubation and the cumulative gas production at end of incubation were increased (P< 0. 05) by the enzyme treatment. Exogenous enzyme had no effects the concentrations of valeric acid and isovaleric acid during incubation (P>0. 05). The results suggested that the exogenous fibrolytic enzymes enhanced the activities of xylanase, endoglucanase and exoglucanase in the fermentation fluid in early incubation, finally increased total volatile fatty acids output and improved rumainal fermentation characteristic in vitro.关键词
纤维素酶/木聚糖酶/挥发性脂肪酸/体外试验Key words
cellulase/ xylanase/ volatile fatty acids/ in vitro分类
农业科技引用本文复制引用
庄苏,丁立人,周建国,王恬..添加外源纤维水解酶对羊草体外瘤胃发酵酶活性及特性的影响[J].草业学报,2013,22(1):315-322,8.基金项目
农业部结构调整重大技术研究专项(20080901B)资助. （20080901B）
