成都中医药大学学报2012，Vol.35Issue(4)：29-32,45,5.
大黄ISSR-PCR反应体系的建立与优化
Establishment and Optimization of ISSR-PCR Reaction System for Rhubarb
摘要
Abstract
An orthogonal design combined with single factor experiment was applied to optimize ISSR-PCR amplification system of Rhubarb. The main elements of the reaction system ( Mg2+ 、 Taq DNA polymerase、dNTPs、 Primer、 DNA template) were tested . The result was analyzed by statistics software and direct analyzing . A suitable ISSR-PCR reaction system was established. Namely 25μL reaction system containing 30 ng DNA template, 2. 5μL 10 x Taq Buffer, 0. 4mmol/L dNTPs, 0. 25μmol/L primer, 2. 5 mmol/ L Mg2+ , 1. 25 U Taq DNA polymerase .关键词
大黄/ISSR-PCR/正交设计/单因素试验Key words
Rhubarb/ISSR-PCR/orthogonal design/single factor experiment分类
医药卫生引用本文复制引用
李敏,齐景梁,蒋彧,孙淑霞,敬勇..大黄ISSR-PCR反应体系的建立与优化[J].成都中医药大学学报,2012,35(4):29-32,45,5.基金项目
国家十一五支撑计划项目(编号:2009BAI84B01) （编号:2009BAI84B01）
