成都中医药大学学报2012，Vol.35Issue(4)：42-45,4.
白芍原药材与不同炮制加工品的高效液相色谱对比研究
Comparative Study on Paeoniae Radix Alba Different Processed Products by High Performance Liquid Chromatography (HPLC)
摘要
Abstract
Objective: The preliminary study in the difference of the chemical composition of different processed products of paeoniae radix alba, in order to provid the basis of the processing of paeoniae radix alba. Methods: Respectively analyzing chemical compositions of paeoniae radix alba medicinal material, white peony root decocting pieces, stir-frying paeoniae radix alba with bran, stir-baked paeoniae radix alba with wine, paeoniae radix alba fermented by HPLC, and comparative study on chemical compositions of the whole finger-print. Using Agilent C18 ( 150 mm×4. 6 mm, 5 μm) column, the mobile phase was composed of a mixture of methyl cyanides -0. 1 % phosphoric acid, The UV detection wavelength was set at 230 nm (content determination) and 210nm (fingerprint) , the column temperature was 30 ℃. Results: the chemical compositions and content of crude and processed paeoniae radix alba are certain differences, and the content of paeoniflorin in stir-baked paeoniae radix alba with wine, paeoniae radix alba fermented has certain increase, and the content of all kinds of chemical composition in paeoniae radix alba fermented is higher than other processed products. Conclusion: In the paeoniae radix alba processing method, the fermentation method should be the best method.关键词
白芍/炮制加工/芍药苷/高效液相色谱指纹图谱Key words
Paeoniae Radix Alba,/ processed/peoniflorin/ HPLC finger-print分类
医药卫生引用本文复制引用
马玉,郭力,黄玉慧,周勤梅,许莉..白芍原药材与不同炮制加工品的高效液相色谱对比研究[J].成都中医药大学学报,2012,35(4):42-45,4.基金项目
科技部科技人员服务企业项目(编号:2009GJF00015) （编号:2009GJF00015）
