重庆医学2013，Vol.42Issue(1)：37-39,3.DOI:10.3969/j.issn.1671-8348.2013.01.012
葛根素与雌二醇对去卵巢大鼠子宫的影响
Effects of puerarin and estradiol on uterus of ovariectomized rats
摘要
Abstract
Objective To observe the effect of long-term combination therapy with puerarin and estradiol on uterine structure of ovariectomized rat .Methods Forty healthy 5-month-old female rats were randomly divided into 5 groups (n= 8):sham-operated , ovariectomized ,puerarin,estradiol and puerarin-estradiol group .Rats in sham-operated group were subjected to removal of ovarian fat pads only ,and ovariectomized-induced osteoporosis rats model were established in the rest 4 groups .One week after modeling , rats in the puerarin group were subcutaneously injected with 50 mg/kg puerarin once daily ,and rats in the estradiol group with 200 jug/kg estradiol twice weekly ,and rats in puerarin-estradiol group with puerarin(25 mg/kg,once daily) and estradiol(100μg/ kg ,twice weekly) simultaneously .After 20 weeks of drug intervention ,serums and uterine tissues of rats in each group were collected,and the level of serum estradiol and morphological structure of uterus were observed.Results Serum estradiol level ,uterine diameter,endometrium area ,myometrium area and uterine gland area of rats in estradiol group were significantly higher than those in ovariectomized ,puerarin and puerarin-estradiol group(P>0.05).Compared with puerarin-estradiol group and puerarin group, rats in the former showed a increasing trend of serum estradiol level ,uterine diameter,endometrium area ,myometrium area and u-terine gland area,with no statistically significant (P>0.05) .Conclusion Both low dose of estradiol and puerarin combination and relatively high dose of peuerarin possess small effect on uterine proliferation .关键词
雌二醇/子宫/大鼠/葛根素Key words
estradiol /uterus /rats /puerarin引用本文复制引用
黄海玲,谭慧欢,赵琼芝,李海,方晓燕..葛根素与雌二醇对去卵巢大鼠子宫的影响[J].重庆医学,2013,42(1):37-39,3.基金项目
广西自然科学基金资助项目(合同编号2010GXNSFA013268). （合同编号2010GXNSFA013268）
