重庆医学2013，Vol.42Issue(1)：46-48,3.DOI:10.3969/j.issn.1671-8348.2013.01.015
16S rRNA基因序列分析鉴定非典型细菌的实验研究
Experimental study of 16S rRNA gene sequence analysis for identification of atypical bacteria
沈亚娟 1夏云1
作者信息
- 1. 重庆医科大学附属第一医院检验科,重庆,400016
- 折叠
摘要
Abstract
Objective To establish an approach for bacteria identification based on 16S rRNA gene sequence analysis ,and apply it to clinical routine bacteria identification initially .Methods Bacteria which can not be accurately identified in clinical microbiologi -cal laboratory were selected .16S rRNA served as target sequence ,primers were designed to match the conserved region at both ends and target fragments were amplified by means of PCR reaction .After sequencing ,their sequences were compared with 16S rRNA sequence of known bacteria in the database .Results Among 13 strains ,11 strains showed sequence similarity of 99 .0% with 16S rRNA sequence of known bacteria in the database ,and were successfully identified to the species level .Conclusion 16S rRNA gene sequence analysis can be applied to identify atypical bacteria quickly and accurately ,and serve as a supplementary method of routine bacteria identification .关键词
基因,rRNA/细菌/序列分析Key words
genes ,rRNA /bacteria /sequence analysis引用本文复制引用
沈亚娟,夏云..16S rRNA基因序列分析鉴定非典型细菌的实验研究[J].重庆医学,2013,42(1):46-48,3.
