重庆医学2013，Vol.42Issue(3)：243-245,3.DOI:10.3969/j.issn.1671-8348.2013.03.002
乳腺癌高表达基因FAM83A的生物信息学分析
Bioinformatics analysis of the highly expressed FAM83A gene in breast cancer*
摘要
Abstract
Objective To screen and find over expressed gene in breast cancer,and to predict the function of FAM83A by bioin formatics. Methods We used digitai gene expression displayer(DGED) tool in cancer genome anatomy project(CGAP) to screen the differentially expressed genes between the breast cancer and benign tumor. The sequence homology,properties,subcellular loca tion, structure and expression profile of FAM83A were analyzed by bioin formatics software. At last,we detected the expression of FAM83A in breast cancer,breast fibroadenoma and normal breast tissue by RT PCR. Results We found a differentially expressed gene FAM83A which had three transcript variants. It was evolutionary conservation. Bioinformatics analysis indicated that FAM83A protein was located likely in the mitochondria. There were PK,PKA phosphorylation sites,MAPK recognition site,SH2, SH3,RPEL and WH2 binding motif. The secondary structure mainly comprised of alpha helices and random coils. RT PCR results confirmed that FAM83A was over expressed in breast cancer tissues,while no expression in benign breast tumor and normal breast tissue. Conclusion FAM83A is over expressed in breast cancer and maybe act as a study target for molecular mechanism.关键词
乳腺肿瘤/生物信息学/SAGE库/FAM83AKey words
breast neoplasms/ bioinformatics/ SAGE library/ FAM83A引用本文复制引用
刘镭,黄亮,车清海,赵恩宏,黄旭,程露阳,肖丽君..乳腺癌高表达基因FAM83A的生物信息学分析[J].重庆医学,2013,42(3):243-245,3.基金项目
河北省人口与计划生育委员会资助项目(2009-B21) （2009-B21）
河北省卫生厅资助项目(20090579). （20090579）
