重庆医学2013，Vol.42Issue(3)：329-331,3.DOI:10.3969/j.issn.1671-8348.2013.03.035
2008～2009年南宁市季节性H1N1亚型流感病毒血凝素HA1基因变异分析
Analysis of the genetic variation of HA1 gene of human seasonal influenza H1N1 virus in Nanning during 2008-2009
摘要
Abstract
Objective To analyze the data of influenza surveillance and discuss the epidemic situation,genetic variation and evolution characteristics of HA1 gene of human influenza H1N1 viruses circulating in Nanning City during 2008 - 2009. Methods 20 H1N1 strains(10 strains each year) isolated in influenza surveillance and epidemic situation during 2008 - 2009 were selected. RNAs were extracted,their HA genes were amplified by RT-PCR and then sequenced. The data obtained were compared with the vaccine strains recommended by WHO with the software VectorNTI 9. 0. Genetic distance and phylogenetic tree of these strains were constructed by using Mega 4. 1. Results Seasonal influenza epidemics mainly occurred in the summer and autumn in Nanning during 2008 - 2009. The HA1 phylogenetic tree constructed and showed that the strains were divided into two branch, both vaccine strains A/Solomon /3/2006 and A/Brisbane/59/2007 were located in branch Ⅰ and Ⅱ , analysis of the H1N1 HA1 amino acid sequence showed that 20 strains disulfide bonds and glycosylation sites were more conserved,but the receptor binding site(RBS) and the antigenic sites were relatively active, including the RBS 188 ,189 ,222,224 occurred in distinct variation. Compared to the vaccine strains A/Solomon /3/2006,the strains in 2008 showed four amino acid substitution which located in two antigenic sites. Except to A/nanning/473/2009 , all strains that isolated in 2009 showed less than 4 variation in antigenic sites. Conclusion The genetic characteristics of HA1 gene of influenza H1N1 virus isolated in Nanning in 2009 was similar to A/Brisbane/59/2007. More genetic variation occurred in 2008 strains compared with two vaccine strains recommended, which may lead to human seasonal influenza epidemics in 2008.关键词
流感,人/H1N1/血凝素类/基因变异Key words
influenza, human/ H1N1 /hemagglutinins/genetic variation引用本文复制引用
范云燕,刘海燕,林新勤,秦剑秋,黄莉,覃巍巍..2008～2009年南宁市季节性H1N1亚型流感病毒血凝素HA1基因变异分析[J].重庆医学,2013,42(3):329-331,3.基金项目
广西南宁科学研究与技术开发计划项目(200802127C). （200802127C）
