重庆医学2013，Vol.42Issue(4)：410-412,3.DOI:10.3969/j.issn.1671-8348.2013.04.019
亚低温减轻沙土鼠海马前脑缺血再灌注损伤和对Hsp70表达的影响
Mild hypothermia alleviating hippocampal CA1 regions forebrain ischemia-reperfusion injury in gerbils and its effects on the expression of Hsp70
摘要
Abstract
Objective To explore the effects of mild hypothermia ( keep the temperature of gerbil eardrum at 33 ℃ for 4 hs) on hippocampal CA1 regions cell injuries in gerbils after forebrain ischemia-reperfusion and the expressions of Hsp70,and detect the possible mechanism of mild hypothermia alleviating brain injury. Methods The experimentation adopted gerbil forebrain ischemia reperfusion injury model induced by 5 min of obstruction in bilateral common carotid artery. The gerbils were randomly divided into 4 groups: sham group(SH) ,hypothermia sham group (HSH) ,normothermic ischemic reperfusion group (IR) and hypothermic is-chemic reperfusion group (HIR). Then the animals were designated as subgroups 2 h,4 h,l d,3 d,and 5 d (n=6 in each) at different time spots after ischemia. Behavioral studies were conducted at predetermined time points. The apoptotic cells in CA1 region were detected by TUNKL method. The variation of Hsp70 in CA1 region was detected by SP immunocytochemical technique. Results The abnormal behaviors and the number of apoptotic cells in hippocampal CA1 region were much less in HIR group than in IR group (P＜0. 01) after 4 h of mild hypothermia. The expression of Hsp70 increased in HIR group compared with that in IR group after 1 d(P＜0. 01). Conclusion 4 h mild hypothermia could increase the expression of Hsp70 in gerbils Cal region after forebrain ischemia-reperfusion,and it may be one of the mechanisms that reduce apoptotic cells and protect the brain.关键词
再灌注损伤/海马/HSP70热休克蛋白质类/沙鼠亚科/亚低温Key words
reperfusion injury/ hippocampus/ HSP70 heat-shock proteins/gerbillinae/ mild hypothermia引用本文复制引用
陈秀侠,滕大才,李军,胡忠浩..亚低温减轻沙土鼠海马前脑缺血再灌注损伤和对Hsp70表达的影响[J].重庆医学,2013,42(4):410-412,3.基金项目
徐州市科技局社会发展资助项目(XF11C064). （XF11C064）
