重庆医学2013，Vol.42Issue(5)：523-525,3.DOI:10.3969/j.issn.1671-8348.2013.05.016
莱菔硫烷对乳鼠心肌细胞缺血缺氧损伤的保护作用
Protective effects of sulforaphane on cardiomyocyte injury induced by ischemia and hypoxia in neonate rat
罗则佳 1陈盛 2何念海 2陈曙光3
作者信息
- 1. 成都军区总医院医务部,成都,610083
- 2. 第三军医大学西南医院儿科,重庆,400038
- 3. 第三军医大学西南医院皮肤科,重庆,400038
- 折叠
摘要
Abstract
Objective To explore the protective effects of sulforaphane on myocardial cells injury induced by ischemia-hypoxia in neonatal rat and its possible mechanism. Methods Cultured neonatal rat cardiomyocytes were divided randomly into four groups: control group,model group,low dose of SFN group(1Oμmol/L) ,and high dose of SFN group(30 μmol/L). Cardiomyocytes model was established in vitro. After treated with SFN for 6 hours, cardiomyocyte apoptosis was detected by AnnexinV. The contents of LDH, MDA, and SOD were tested by ELISA. The expression of NF-KB protein in cardiomyocytes was determined by Western Blot. Results After treatment by SFN,the apoptosis cells reduced,the contents of LDH and MDA decreased,and SOD content increased significantly. The level of NF-KB expression decreased, which was more significant in high concentrations. Conclusion SFN can protect myocardial cells from anoxia and ischemic injury,and the mechanism may relate to anti-oxidation effects of SFN and inhibition of NF-KB.关键词
肌细胞,心脏/缺血缺氧/抗氧化/莱菔硫烷Key words
myocytes, cardiac/ ischemia/ hypoxia/ antioxidation/ sulforaphane引用本文复制引用
罗则佳,陈盛,何念海,陈曙光..莱菔硫烷对乳鼠心肌细胞缺血缺氧损伤的保护作用[J].重庆医学,2013,42(5):523-525,3.
