重庆医学2013，Vol.42Issue(7)：765-767,3.DOI:10.3969/j.issn.1671-8348.2013.07.019
机械和感染双重损伤法建立新西兰大白兔宫腔粘连模型
Establishment of New Zealand white rabbit intrauterine adhesions model by mechanical and infectious double injury
摘要
Abstract
Objective To construct a rabbit model of intrauterine adhesions(IUA) to benefit the deepening study of intrauterine adhesions pathogenesis. Methods Sixty New Zealand female rabbits were randomly divided into the control group,mechanical inju ry group,infection injury group and double injury group,15 cases in each group. The mechanical injury group was treated with cu rettage of uterine,the infection injury group with intrauterine indwelling lipopolysaccharide cotton for 2 d,the double damage group with indwelling lipopolysaccharide cotton for 2 d after curettage for injuring endometrium and the control group was performed the sham operation. In order to dynamically observe the endometrial pathological changes process,the uterine tissues were collected re spectively before modeling,on 2,7,14,28 d after modeling and performed HE and Masson staining. The number of endometrial glands and the ratio of fibrosis area on 7 d after modeling in each group were analyzed by statistical software. Results The ratio of fibrosis area in the double injury group was 0.743±0.066 and higher than 0. 359±0. 061 4 and 0. 348 ± 0. 086 in the mechanical in jury group and the infection group respectively,showing statistical differences between them(P<0. 05). The number of endometrial glands in the double injury group was 4. 190 ± 1. 350 and lower than 12. 660 ± 1. 470 and 13. 590 ± 1. 560 in other two groups with statistical differences(P<0. 05). Conclusion The double damage method of mechanical injury and infection can establish stable rabbit uterine adhesion models.关键词
宫腔粘连/动物模型/损伤/纤维化Key words
intrauterine adhesions/animal models/injury/fibrosis引用本文复制引用
刘芳,何援利..机械和感染双重损伤法建立新西兰大白兔宫腔粘连模型[J].重庆医学,2013,42(7):765-767,3.基金项目
国家自然科学基金资助项目(81270658). （81270658）
