大豆科学2013，Vol.32Issue(1)：23-27,5.
RAV基因对拟南芥和大豆不定芽再生的影响
Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean
摘要
Abstract
RAV is a member of the ERF/AP2 transcription factor family. We use explants of Arabidopsis rav T-DNA insertion homozygous mutants and inhibition (-i) of GmRAV in soybean as experimental material to carry out callus induction. Various concentration of exogenous Cytokinins 2-ip and 6-BA were added in the shoot-inducing medium and the changes in the average number of shoots and the shoot regeneration rates were investigated. It turned out that Cytokinins had positive effects on the shoot regeneration, in addition, the average number of shoots and shoot regeneration rates of Arabidopsis rav mutants and Gm-RAV-i soybean plants would decrease by 2-ip and 6-BA. It proved that the function of RAV homologs was conserved and RAV promoted shoot regeneration in the presence of Cytokinins.关键词
大豆/GmRAV/拟南芥/不定芽再生/细胞分裂素Key words
Soybean/GmRAV/Shoot regeneration/Cytokinins分类
农业科技引用本文复制引用
卢清瑶,赵琳,李冬梅,谷月娇,张彦威,吕庆雪,刘丽雪,李文滨..RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(1):23-27,5.基金项目
国家自然科学基金(31101169,30671318,31271748) （31101169,30671318,31271748）
转基因生物新品种培育重大专项(2011ZX08004-005) （2011ZX08004-005）
黑龙江省教育厅大豆分子设计团队项目 （）
黑龙江省教育厅省高校青年学术骨干项目 （）
东北农业大学博士基金. （）
