大豆科学2013，Vol.32Issue(1)：136-138,3.
卡那霉素和草铵膦对不同基因型大豆胚尖不定芽诱导的影响
Effect of Knamycin and Glufosinate on Adventitious Buds Induction from Embryonic Tip of Different Genotype Soybean
摘要
Abstract
Embryonic tips of Hefeng35, Heinong44 and Jilin35 were used as the explants to investigate the effects of kanamy-cin and glufosinate on the adventitious bud induction. Under 100 mg?L-1 kanamycin, the adventitious bud induction rate of Hefeng35 and Jilin35 were not significantly different compared with control, while Heinong44 was significantly lower than control; bud number of Jilin35 was lower than control and higher than other concentrations, Hefeng35 and Heinong44 were not different compared with control;the shoot length of 3 genotypes were significantly inhibited by 100 mg?L-1 kanamycin. Glufosinate at the concentration from 0.2 to 1.2 mg?L-1 had no influence on the number of adventitious buds for Hefeng35 and Ji-Iin35,but Heinong44 reduced conspicuously at the concentration of 1.0 mg?L-1. Both Heinong44 and Jilin 35 were inhibited by 0.2 mg?L-1 glufosinate at length of buds while Hefeng35 was inhibited by 0.6 mg?L-1 glufosinate. Results suggest the suitable screening concentration of kanamycin for Hefeng35, Heinong44 and Jilin35 were 100 mg ? L-1, and glufosinate for three genotypes were 0.6,0.2 and 0.2 mg?L-1,respectively.关键词
大豆/胚尖/不定芽/卡那霉素/草铵膦Key words
Soybean/Embryonic tips/Adventitious bud/Knamycin/Glufosinate分类
农业科技引用本文复制引用
张忻爽,王萍,宋海星,荣湘民..卡那霉素和草铵膦对不同基因型大豆胚尖不定芽诱导的影响[J].大豆科学,2013,32(1):136-138,3.基金项目
国家转基因生物新品种培育重大专项(2009ZX08010-013B) （2009ZX08010-013B）
中央财政支持地方高校发展专项资金(CXTD07). （CXTD07）
