大连工业大学学报2012，Vol.31Issue(6)：405-408,4.
超声波破碎法提取发酵性丝孢酵母胞内脂肪酶的条件优化
Optimization of ultrasonic disruption for lipase extraction from Trichosporon fermentans
王迪 1曹方 1栾静 1孙玉梅1
作者信息
- 1. 大连工业大学 生物工程学院,辽宁 大连 116034
- 折叠
摘要
Abstract
The disruption and lipase release of Trichosporon fermentans by ultrasonication was examined. The effect of ultrasound output power, duration of irradiation each time, total irradiation time and the cell concentration on the activity of lipase were investigated through the single-factor and orthogonal experiment. The lipase activity reached 128.46 U/g under the optimum extraction condition: duration of irradiation each time 5 s (with a 5 s interval), the cell concentration 25 g/L, output power 450 W, total irradiation time 5. 5 min.关键词
超声波/发酵性丝孢酵母/细胞破碎/脂肪酶Key words
ultrasonication/ Trichosporon fermentans/ cell disruption/ lipase分类
轻工纺织引用本文复制引用
王迪,曹方,栾静,孙玉梅..超声波破碎法提取发酵性丝孢酵母胞内脂肪酶的条件优化[J].大连工业大学学报,2012,31(6):405-408,4.
