动物学研究2013，Vol.34Issue(1)：39-46,8.DOI:10.3724/SP.J.1141.2013.01039
脊尾白虾组织蛋白酶L基因的克隆及其表达分析
Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda
摘要
Abstract
Based on the EST sequence from a hemocyte cDNA library, the cathepsin L cDNA of Exopalaemon carinicauda (EcCatL) was cloned by rapid amplification of cDNA ends (RACE). The EcCatL cDNA was 1136 bp in length, which contains an open reading frame (ORF) of 960 bp, encoding a 319 amino-acid polypeptide. Homology analysis revealed that the amino acid sequence of EcCatL was highly conserved with its homologs in other crustaceans. The similarities of EcCatL with the CatL of Palaemonetes varians and Pandalus borealis were 92% and 76%, respectively. Phylogenetic analysis showed that EcCatL was in the same branch as that of Palaemonetes varians. The expression levels of EcCatL in different tissues were analyzed by quantitative real-time PCR. Expression of EcCatL was detected in all tested tissues of E. carinicauda, including hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach and eyestalk, with the highest expression level in hepatopancreas. After challenged with Vibrio anguillarum or white spot syndrome virus, the expression of EcCatL were up-regulated in the hemocytes and hepatopancreas of E. carinicauda. Our results implied that EcCatL might play an important role in the prawn immune response.关键词
脊尾白虾/组织蛋白酶L/基因克隆/基因表达Key words
Exopalaemon carinicauda/ Cathepsin L/ Gene cloning/ Gene expression分类
生物科学引用本文复制引用
段亚飞,刘萍,李吉涛,李健,高保全,陈萍..脊尾白虾组织蛋白酶L基因的克隆及其表达分析[J].动物学研究,2013,34(1):39-46,8.基金项目
国家高技术研究发展计划课题(2012AA10A409) （2012AA10A409）
国家虾产业技术体系(CARS-47) （CARS-47）
公益性行业(农业)科研专项(201103034) （农业）
中国水产科学研究院基本科研业务费资助(2013A0701) （2013A0701）
