动物营养学报2012，Vol.24Issue(12)：2415-2423,9.DOI:10.3969/j.issn.1006-267x.2012.12.016
谷氧还蛋白1和硫氧还蛋白1基因在云南乌金猪不同组织中的表达特点及L-组氨酸对其在氧化应激细胞中表达的影响
Glutaredoxin 1 and Thioredoxin 1 : Gene Expression Characteristics in Different Tissues and Effects of L-Histidine on Gene Expressions in Oxidant Stress Cells of Yunnan Wujin Pigs
摘要
Abstract
This experiment was conducted to investigate the gene expressions of glutaredoxin 1 ( GRX1) and thioredoxin 1 ( TRX1) in pituitary, hypothalamus, thyroid, thymus, pancreas, gonad, liver, skin, duode-num , jejunum and ileum tissues and explore the regulation of L-histidine on gene expressions in oxidative stress cells of Yunnan Wujin pigs. Different expressions of GRXl and TRXl genes were determined by the method of real-time quantitative PCR (RT-qPCR) , and the regulation of L-histidine on GRX1 and TRX1 gene expressions was explored in oxidative stress cells induced by H2 O2. The results showed as follows: 1) GRX1 and TRX1 gene expressions in tissues of Wujin pigs were detected, the highest gene expressions of GRX1 and TRX1 were in the liver, and then skin and jejunum, and relatively lower gene expressions were in the other tissues. 2) TRX1 gene expression in tissues was higher than GRX1 gene expression for Wujin pigs. 3) The results of cell culture showed that when being induced by H2O2, oxidative stress cells produced the over expression of GRX1 and TRX1 genes. L-histidine had the regulation on GRX1 and TRX1 gene expressions in oxidative stress or no stress cells, being the adequate concentration of 280 μg/mL. GRX1 and TRX1 gene expressions had the obvi-ous tissue characteristics for Wujin pigs. H2O2 could induce the over expression of GRX1 and TRX1 genes in oxidative stress cells. Adding adequate concentration of L-histidine could regulate GRX1 and TRX1 gene ex-pressions in oxidative stress or no stress cells of Wujin pigs. It is suggested that this may be an important way of reducing the oxidative stress damage and improving the oxidation resistance, being GRX1 and TRX1 gene expressions of animal body induced by nutritional pathway for Wujin pigs.关键词
乌金猪/氧化应激/谷氧还蛋白1/硫氧还蛋白1/基因表达/L-组氨酸Key words
Wujin pigs/ oxidative stress/ GRX1/ TRX1/ gene expression/ L-histidine分类
农业科技引用本文复制引用
张春勇,陈克嶙,黄金昌,郭荣富..谷氧还蛋白1和硫氧还蛋白1基因在云南乌金猪不同组织中的表达特点及L-组氨酸对其在氧化应激细胞中表达的影响[J].动物营养学报,2012,24(12):2415-2423,9.基金项目
云南现代农业生猪产业技术体系-生猪营养(A3006688) （A3006688）
